Bio-Rad Profinity IMAC Resins - Manual (Page 13)

Table 4. Buffer Composition
KCl KH
2
PO
4
Imidazole Urea
Native lysis/
wash buffer 1 300 mM 50 mM 5 mM N/A
Native wash
buffer 2 300 mM 50 mM 10 mM N/A
Native elution buffer 300 mM 50 mM 250 mM N/A
Denaturing lysis/
wash buffer 1 300 mM 50 mM 5 mM 6M
Denaturing wash
buffer 2 300 mM 50 mM 10 mM 6M
Denaturing elution
buffer 300 mM 50 mM 250 mM 6M
Table 5. Buffer Formulations
KCl KH
2
PO
4
Imidazole Urea
Native lysis/wash
buffer 1 22.37 g 6.80 g 0.34 g N/A
Native wash
buffer 2 22.37 g 6.80 g 0.68 g N/A
Native elution buffer 22.37 g 6.80 g 17.02 g N/A
Denaturing lysis/
wash buffer 1 22.37 g 6.80 g 0.34 g 360.36 g
Denaturing wash
buffer 2 22.37 g 6.80 g 0.68 g 360.36 g
Denaturing elution
buffer 22.37 g 6.80 g 17.02 g 360.36 g
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