Bio-Rad Profinity IMAC Resins - Manual (Page 17)

~30% expression ~25% expression
Soluble partitioning Insoluble partitioning
Fig. 2. Partioning profiles. For both gels, Precision Plus Protein™
molecular weight markers were loaded in lane 1, followed by the total, soluble,
and insoluble fractions in lanes 2–4 respectively. The first panel depicts a
32 kD target protein, which partitions into the soluble fraction and can be
purified using the native protocol (outlined on page 15). A representative
chromatogram and gel for the purification of this target protein is shown in
Fig. 5 on page 20. The second panel depicts a 24 kD target protein, which
partitions into the insoluble fraction and can be purified using the denaturing
protocol (outlined on page 16).
14
1 2 3 4 1 2 3 4
kD
250
150
100
75
50
37
25
20
15
10
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