Bio-Rad Nuvia™ IMAC Resin - Manual (Page 9)

Nuvia IMAC Ni-Charged Resin 5
Chemical Compatibilities
The chemical characteristics of Nuvia IMAC resin are detailed in Table 2.
Table 2. Chemical Compatibilities for Nuvia IMAC Resins.
Reagent
Group
Reagent Comments Stability
Buffer
reagents
Tris, HEPES,
MOPS
Sodium or
potassium
phosphate
Used with
proteins more
stable in
nonphosphate
buffers
≤50 mM
secondary and
tertiary amines
50 mM sodium
or potassium
phosphate are
recommended
as starting
buffers
Chelating
reagents
EDTA, EGTA Strips nickel ions
from the resin
≤0.1 mM
successfully
used to remove
trace metal
contaminants
>1 mM can
cause significant
reduction in
binding capacity
Sulfhydryl
reagents
β-Mercaptoethanol
DTT, TCEP
Reduces random
disulfide bonds
preventing
protein
aggregation
during purification
Transition metals
at the center of
IMAC resin (Ni 2+ )
are susceptible to
reduction
≤20 mM
≤10 mM DTT
and 20 mM
TCEP
Detergents Nonionic
detergents (Triton,
Tween)
Zwitterionic
detergents
(CHAPS, CHAPSO)
Removes
background
proteins
and nucleic acids
Solubilizes
membrane
proteins
≤2%
≤1%
Denaturants Guanidine HCl
(GuHCl)
Urea
Solubilizes
proteins
≤6 M
≤8 M