Bio-Rad Nuvia™ IMAC Resin - Manual (Page 10)

6 Nuvia IMAC Ni-Charged Resin
Table 2. Chemical Compatibilities for Nuvia IMAC Resins, Continued.
Reagent
Group
Reagent Comments Stability
Other
additives
NaCl Deters
nonspecific
protein binding
due to ionic
interactions
≤2 M (at least
300 mM NaCl
should be
included in
buffers)
MgCl
2
Essential
component for
purification of
Ca 2+ binding
proteins
≤100 mM
(HEPES or Tris
buffers should be
used to prevent
precipitation)
CaCl
2
Essential
metal cofactor for
nucleases
≤5 mM (HEPES
or Tris buffers
should be used
to prevent
precipitation)
Glycerol Included
to prevent
hydrophobic
interactions
between proteins
≤20%
(backpressure
may increase
significantly,
slower flow rates
may be required)
Ethanol Included
to prevent
hydrophobic
interactions
between proteins
≤20%
Imidazole Competes for
binding sites with
histidine-tagged
residues by
interaction
with the metal
residues
May be
used in low
concentrations
in the wash
buffer (<30 mM)
to limit binding
of undesired
proteins; for
elution, ≤500 mM
may be used