Bio-Rad Nuvia™ IMAC Resin - Manual (Page 15)

Nuvia IMAC Ni-Charged Resin 11
Section 4
Column Packing — Medium-Pressure Columns
Slurry Packing an IMAC Column
Slurry packing is preferred for small columns. This method
describes packing a Nuvia
™ IMAC resin with aid from a pump. For
best results, use 5–50 mm ID columns and a bed height of
5–30 cm.
Recommended Columns
Bio-Rad’s Bio-Scale ™ MT high-resolution columns may be used
for the following column-packing procedure. These columns are
empty and may be packed with the support of choice.
Bio-Scale MT columns are convenient to use with Bio-Rad’s
NGC
™ chromatography system or any medium- or high-pressure
system:
• Bio-Scale MT2 column (7 x 52 mm) for bed volumes
up to 2 ml
• Bio-Scale MT5 column (10 x 64 mm) for bed volumes
up to 5 ml
• Bio-Scale MT10 column (12 x 88 mm) for bed volumes
up to 10 ml
• Bio-Scale MT20 column (15 x 113 mm) for bed volumes
up to 20 ml
Materials
• Empty column (1–5 cm ID x 30 cm) with flow adaptors, inlet
and outlet ports
• Glass filter
• Nuvia IMAC Ni-charged resin
• Packing reservoir
• Pump