Bio-Rad Nuvia™ IMAC Resin - Manual (Page 19)

Nuvia IMAC Ni-Charged Resin 15
Method
1. Thoroughly suspend Nuvia IMAC resin.
2. Place the column into an appropriate collection vessel; for
example, a 2 ml capless collection tube.
3. Using a pipet, transfer the appropriate amount of Ni-charged
Nuvia IMAC resin to a microcentrifuge tube. If using a Micro
Bio-Spin column, transfer 0.2 ml slurried Ni-charged Nuvia
IMAC resin to the column. This is equivalent to ~100 µl of a
packed resin bed.
4. Remove storage solution by centrifugation. Centrifuge at
1,000 x g for 15 sec to pack resin.
5. Wash column with at least 5 column volumes (or ~500 µl) of
distilled water. Centrifuge at 1,000 x g for 15 sec to pack resin.
6. If using Ni-charged resin, equilibrate the column with at least
5 column volumes of binding buffer. The column is now ready
for separation.