Bio-Rad Nuvia™ IMAC Resin - Manual (Page 3)

Table of Contents
Section 1 Introduction ............................................................ 1
Section 2 Product Description ............................................... 2
Nuvia ™ IMAC Resins and
UNOsphere ™ Technology .......................................... 2
Chemical Interactions ............................................... 3
Resin Characteristics ................................................ 4
Chemical Compatibilities ........................................... 5
Section 3 General IMAC Procedures ..................................... 7
Protein Binding ......................................................... 7
Washes .................................................................... 8
Elution ...................................................................... 8
Purification under Denaturing Conditions .................. 9
Imidazole Concentrations ........................................10
Section 4 Column Packing — Medium-Pressure
Columns .................................................................11
Slurry Packing an IMAC Column...............................11
Recommended Columns..........................................11
Materials ..................................................................11
Resin Preparation ....................................................12
Method ................................................................... 12
Section 5 Column Packing — Sample Preparation–Sized
Columns ................................................................ 14
Materials ................................................................. 14
Resin Preparation ................................................... 14
Method ...................................................................15
Section 6 Immobilizing Metal Ions ........................................16
Section 7 Medium-Pressure Column Purification of
Histidine-Tagged Proteins....................................17
Materials .................................................................17
Method ...................................................................18
Materials and Method for On-Column
Renaturation ........................................................... 20