Bio-Rad Nuvia™ IMAC Resin - Manual (Page 8)

4 Nuvia IMAC Ni-Charged Resin
Resin Characteristics
The characteristics of Nuvia IMAC resin are detailed in Table 1.
Table 1. Characteristics of Bio-Rad Nuvia IMAC Resin.
Functional ligand Nitrilotriacetic acid (NTA)
Base bead UNOsphere base matrix
Form 50% suspension in 20% ethanol or pre-
packed into columns; comes precharged
with Ni
2+
Particle size 38–53 µm
Mean particle size 50 µm
Metal ion capacity ≥18 µmol Cu 2+ /ml Nuvia IMAC resin
Dynamic binding
capacity* ≥40 mg/ml resin
Recommended linear
flow rate <500 cm/hr at 25°C
Maximum operating
pressure 45 psi
pH stability, short-term/
cleaning 2–14
Chemical compatibility See Table 2
Storage 4°C to ambient temperature
Shelf life in 20% ethanol ≥3 year at ambient temperature
Operational temperature 4–40°C
Autoclaving conditions 0.1 M sodium acetate at 120°C for 30 min
* Dynamic binding capacity conditions (Q10% determination):
Column volume:
1 ml, 5.6 mm x 4 cm (ID x H)
Sample: 1.0 mg/ml 6x histidine-tagged pure protein (40 kD)
Note: Dynamic binding capacity will vary from protein to protein.