Bio-Rad Nuvia™ IMAC Resin - Manual (Page 18)

14 Nuvia IMAC Ni-Charged Resin
Section 5
Column Packing — Sample Preparation–Sized
Columns
Use this method for packing Nuvia ™ IMAC resin into sample
preparation micro spin columns.
Materials
1. Reagents
• 0.1 M NiSO
4
(or other suitable metal salt solution)
Note: Charging Nuvia IMAC nickel-charged resin (Ni-charged
resin) is not required for initial use. For subsequent uses it is
recommended that the resin be cleaned of all contaminants,
stripped of metal ions, and recharged with proper metal ions
prior to loading sample.
If needed, the procedure to charge Nuvia IMAC (uncharged)
resin is explained in Section 6, Immobilizing Metal Ions.
2. Equipment
• Sample preparation–sized columns (for example,
Micro Bio-Spin ™ columns, catalog #732-6204)
• Plasticware, 2 ml capped and 2 ml capless tubes
• Nuvia IMAC Ni-charged resin
• Tabletop centrifuge
• 1 ml pipet with wide-bore pipet tips
Resin Preparation
Nuvia IMAC Ni-charged resins come in a 20% ethanol solution,
supplied for resin storage. The column can be packed in this slurry
or decanted into distilled water and packed.