Bio-Rad Aminex Organic Acid and Alcohol Analysis Columns - Manual (Page 2)

Table of Contents
Section 1 Introduction to Resin-Based HPLC Columns ....1
Section 2 Column Set-Up ................................................2
2.1 Unpacking ..................................................................2
2.2 Preparing the Eluant ....................................................2
Section 3 Guard Columns ................................................2
3.1 Installing Guard Columns ............................................3
3.2 Purging the Guard Columns ........................................3
Section 4 Attaching the Column ......................................4
Section 5 Operating Parameters ......................................5
5.1 Flow Rate ....................................................................5
5.2 Sample Preparation ....................................................6
5.3 Pressure Checks ........................................................6
5.4 Column Testing............................................................7
Section 6 Regeneration Procedures ..............................14
6.1 Fluffing the Resin Bed ................................................14
6.2 Cleaning a Contaminated Column..............................14
6.3 Reconverting a Column to its Original Ionic Form ......15
6.4 Topping Off a Column Bed ........................................15
6.5 Rectifying Instrument/Connecting Tubing Problems ..16
Section 7 Heated Column Shut-Down ............................16
Section 8 Column Storage ..............................................17
8.1 Long Term Storage....................................................17
8.2 Short Term Storage ..................................................17
Section 9 Troubleshooting..............................................17
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