Bio-Rad Aminex Organic Acid and Alcohol Analysis Columns User Manual
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Table 2. Cleaning, Regeneration, and Storage Guidelines (continued)
Fermentation
Aminex Aminex
Monitoring Column,
HPX-72-O Column,
HPX-72S Column,
300 x 7.8 mm
300 x 7.8 mm
300 x 7.8 mm
Organic modifier
CH
3
CN 40%;
CH
3
CN 30%;
CH
3
CN 40%;
((maximum)
EtOH, IPA, 5%
EtOH, IPA, 5%
EtOH, IPA, 5%
Inorganic modifier
Phosphoric acid,
NaOH, KOH
Sulfate salts
nitric acid (<5%)
Avoid
MeOH, salts, bases,
MeOH, anions different
MeOH, anions different
metal ions, amines,
than ionic form
than ionic form
other organic solvents,
H
2
O >pH 3
Cleaning
(1) 5% CH
3
CN in 0.005
(1) 5% CH
3
CN in 0.005
(1) 5% CH
3
CN in 0.005
Solvent
M H
2
SO
4
; (2) 30% CH
3
M H
2
SO
4
; (2) 30% CH
3
M H
2
SO
4
; (2) 30% CH
3
CN in 0.005 M H
2
SO
4
CN in 0.005 M H
2
SO
4
CN in 0.005 M H
2
SO
4
Flow rate
0.2 ml/min
0.2 ml/min
0.2 ml/min
Temperature
65°C
25°C
25°C
Duration
(1) 4 hr; (2) 12 hr;
(1) 4 hr; (2) 6 hr
(1) 4 hr; (2) 6 hr
(3) run mobile phase
until steady baseline
Regeneration
0.025 M H
2
SO
4
0.2 M NaOH
0.5 M (NH
4
)
2
SO
4
,
Solvent
pH 9
Flow rate
0.2 ml/min
0.2 ml/min
0.2 ml/min
Temperature
65°C
25°C
25°C
Duration
4–16 hr
4–16 hr
4–16 hr
Shipping/storage
0.005 M H
2
SO
4
0.05 M NaOH
0.1 M (NH
4
)
2
SO
4
Solvent
Lit42D.qxd 11/29/2005 2:23 PM Page 12