Bio-Rad Aminex Organic Acid and Alcohol Analysis Columns - Manual (Page 12)

Table 2. Cleaning, Regeneration, and Storage Guidelines
Aminex Aminex Aminex
HPX-87C Column, HPX-42C Column, HPX-87P Column,
300 x 7.8 mm 300 x 7.8 mm 300 x 7.8 mm
Organic modifier Acetonitrile 30%; Acetonitrile 30%; Acetonitrile 30%;
(maximum) EtOH, IPA, 5% EtOH, IPA, 5% EtOH, IPA, 5%
Inorganic modifier Calcium sulfate or nitrate Calcium salts Lead nitrate
Avoid MeOH, acids, bases, MeOH, acids, bases, MeOH, acids, other salts,
other salts other salts anions that form
insoluble ppts with lead
Cleaning 30% CH
3
CN in water 30% CH
3
CN in water 30% CH
3
CN in water
Solvent
Flow rate 0.2 ml/min 0.2 ml/min 0.2 ml/min
Temperature 25°C 25°C 25°C
Duration 4 hr 4 hr 4 hr
Regeneration 0.1 M Ca(NO
3
)
2
0.1 M Ca(NO
3
)
2
30% acetonitrile;
Solvent 0.1 M Pb(NO
3
)
2
, pH 4.0*
Flow rate 0.2 ml/min 0.2 ml/min 0.2 ml/min
Temperature 85°C 85°C 60°C
Duration 4-16 hr 4-16 hr 16 hr
Shipping/storage Water Water Water
Solvent
*Adjust Pb(NO
3
)
2
to pH 4.0 with 0.1 M HNO
3
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Lit42D.qxd 11/29/2005 2:23 PM Page 10