Bio-Rad Aminex Organic Acid and Alcohol Analysis Columns User Manual
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13
Table 2. Cleaning, Regeneration, and Storage Guidelines (continued)
Aminex Aminex
Aminex
Glyphosate
HPX-87N Column,
HPX-87K Column,
HPX-87C Column,
Analysis Columns,
300 x 7.8 mm
300 x 7.8 mm
250 x 4 mm
100-300 x 7.8 mm
CH
3
CN 30%;
CH
3
CN 30%;
CH
3
CN 40%;
CH
3
CN 30%;
EtOH, IPA, 5%
EtOH, IPA, 5%
EtOH, IPA, 5%
EtOH, IPA, 5%
Na
2
SO
4
K
2
SO
4
Calsium sulfate or
KH
2
PO
4
nitrate
MeOH, acids, bases,
MeOH, acids, bases,
MeOH, acids, bases,
Bases, metal ions
30% CH
3
CN in
30% CH
3
CN in
30% CH
3
CN in water
0.005 M Trisodium citrate;
0.01 M Na
2
HPO
4
0.01 M K
2
HPO
4
0.005 M Tetrasodium ethylene
diaminetetraacetate;
4% MeOH, pH 10.0 (NaOH)
0.2 ml/min
0.2 ml/min
0.2 ml/min
0.2 ml/min
25°C
25°C
25°C
50°C
4 hr
4 hr
4 hr
4 hr
(3) run mobile phase
0.020 M Na
2
HPO
4
,
0.020 M K
2
HPO
4
,
0.1 M Ca (NO
3
)
2
0.005 M K
2
HPO
4
,
pH 9
4% MeOH
0.2 ml/min
—
0.2 ml/min
0.2 ml/min
85°C
85°C
85°C
50°C
4–16 hr
4–16 hr
4–16 hr
16 hr
0.01 M Na
2
HPO
4
0.01 M K
2
HPO
4
Water
0.005 M KH
2
PO
4
+ 4% MeOH
Lit42D.qxd 11/29/2005 2:23 PM Page 13