Bio-Rad Aminex Organic Acid and Alcohol Analysis Columns - Manual (Page 18)

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6.5 Rectifying Instrument/Connecting Tubing Problems
Many instances of poor resolution are not the result of column problems, but rather
the result of hardware problems elsewhere in the system. The following list can
help to point out troublesome areas and provide ideal specifications.
1. Injector
Sample volume 100 µl maximum
Loop volume 500 µl maximum
2. Connecting Tubing
Fittings not deteriorated
Tube end cut square
Tube bottoms in fitting
Tube ID
0.013” maximum
Tube length 10” maximum
3. Detector
Clean cells
Cell inlet tubing 0.013” ID maximum
Column connected to sample side, not reference side
Temperature stable
Section 7
Heated Column Shut-Down
After a high temperature separation, reduce the flow rate to 0.2 ml/min and turn off
the column heater. Continue to pump solvent until the column returns to ambient
temperature. Removing a hot column from the system will cause the solvent to
contract while cooling and air will be pulled into the column.
If a fast change between two columns is required, the hot column can be
immersed in filtered, fresh distilled deionized water to cool rapidly. Remember to
replace the end screws tightly to prevent the resin from drying out.
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