Bio-Rad Aminex Organic Acid and Alcohol Analysis Columns - Manual (Page 13)

11
Table 2. Cleaning, Regeneration, and Storage Guidelines (continued)
Fast Carbohydrate Aminex Aminex Fast Acid
Analysis Column, HPX-42A Column, HPX-87H Column, Analysis Column,
100 x 7.8 mm 300 x 7.8 mm 300 x 7.8 mm 100 x 7.8 mm
Acetonitrile 30%; Acetonitrile 30%; CH
3
CN 40%; CH
3
CN 40%; EtOH,
EtOH, IPA, 5% EtOH, IPA, 5% EtOH, IPA, 5% EtOH, IPA, 5%
Lead nitrate None Phosphoric acid, Phosphoric acid,
nitric acid (<5%), HCl nitric acid (<5%), HCl
MeOH, acids, bases, MeOH, acids, bases, MeOH, salts, bases, MeOH, salts, bases,
other salts all salts, metal ions, amines, metal ions, amines,
other organic solvents, other organic solvents,
H
2
O >pH 3 H
2
O >pH 3
30% CH
3
CN in water 30% CH
3
CN in water (1) 5% CH
3
CN in 0.005 (1) 5% CH
3
CN in 0.005
M H
2
SO
4
; (2) 30% CH
3
M H
2
SO
4
; (2) 30% CH
3
CN in 0.005 M H
2
SO
4
CN in 0.005 M H
2
SO
4
0.2 ml/min 0.2 ml/min 0.2 ml/min 0.2 ml/min
25°C 25°C 65°C 65°C
4 hr 4 hr (1) 4 hr; (2) 12 hr; (1) 4 hr; (2) 12 hr;
(3) run mobile phase (3) run mobile phase
until steady baseline until steady baseline
30 % acetonitrile; Not recommended 0.025 M H
2
SO
4
0.025 M H
2
SO
4
0.1 M Pb(NO
3
)
2
, pH 4.0*
0.2 ml/min — 0.2 ml/min 0.2 ml/min
60°C — 65°C 65°C
16 hr — 4–16 hr 4–16 hr
Water Water 0.005 M H
2
SO
4
0.005 M H
2
SO
4
Lit42D.qxd 11/29/2005 2:23 PM Page 11