Bio-Rad Gel Doc™ XR+ System - Manual (Page 24)

Page 24 of 42
Note: No lights should be turned ON. Please allow approximately 30 seconds for the
Gel Doc
 XR and 30 minutes for the acquisition of the Dark Reference files
ChemiDoc  XRS systems.
1. After the “Dark Reference” acquisition is over the system will be ready to operate.
2. Turn ON the Epi-White lights using the Epi-White touch pad button.
3. Place the focusing target on the transilluminator platen.
4. Click on Live/Focus.
5. An image of the target will be seen on the imaging screen.
6. Using the lens control buttons on the membrane touch pad or the control panel that appears
in the Quantity One  acquisition window, adjust the Iris, Focus, and Zoom.
7. Follow the procedure below to align the Camera with respect to the center of the platen.
Note: For additional details on Dark Reference Acquisition refer to the Quantity One 
Software Manual.
4.3.3 Aligning the Camera assembly:
Centering the CCD Camera on the opening in the top of the Universal Hood II should provide
good alignment of the Camera with the center of the UV transilluminator. To verify, please
follow the instructions below:
1. Place the focusing target in the center of the transilluminator.
2. Switch on the Epi-White light.
3. Open the acquisition window in Quantity One
. Select Live/Focus mode so that a live
image of the target is seen on the monitor.
4. Focus on the target.
5. Click on “Show Alignment Grid” button located near Live/Focus button. A red cross hair
will appear in the middle of the imaging area.
6. Loosen the bracket screw and move it slightly until the black square of the target matches
with the red X-mark in the image. Tighten the screw to secure the Camera in place.
7. Select “Fast” on the hood. Press “Focus” button to confirm that the lens is responding. If
not, adjust the position of the lens so that the opening on the hood offers no
resistance/binding to the focus ring on the lens. This may not allow exact center of the
Target to the image.
8. Use the zoom button on the control panel to zoom in and out on the image.
9. When the Camera assembly and target are correctly aligned, the center of the target will
move a few millimeters in the center of the image throughout the zoom range.