Bio-Rad Fluorescent Protein Stains - Manual (Page 7)

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2.3 Fix Gels
Remove the gel(s) from the gel cassette or plates. Place gel in a clean glass tray
with the volume of fix solution indicated above. Cover the tray, place on a rocker
or shaker and agitate gently. Fix for at least 2 hours.
Note: Gels may be left in fix solution for up to 24 hours. Shortened fix time or
insufficient fix solution may reduce sensitivity.
2.4 Prepare Working (1x) Stain Solution
Working (1x) stain solution is prepared by diluting one volume of Flamingo
fluorescent gel stain with 9 volumes of distilled or deionized water that has been
filtered. The quantity of stain solution required depends on the number of gels to
be stained and the size of the gel as indicated in the table below.
Working (1x) stain solution should be prepared within one day of its intended use.
2.5 Stain Gels
Carefully pour off the fix solution and add Flamingo fluorescent gel stain to the
staining tray. Cover the tray, place on a rocker or shaker and agitate gently. Stain
for at least 3 hours.
Gels may be left in the stain solution for extended periods. If the gel is to be left in
the stain solution for longer than 8 hours, cover the gel tray with aluminum foil or
other opaque material to limit light exposure.
Stained gels may be stored for up to six months and imaged without significant
loss of sensitivity. Gels held for long-term storage should be placed in sealable
plastic bags with 5–10 ml of stain solution and stored in the dark at 2–8°C.
Gel Size
Volume of Stain Solution
per Gel
Ready Gel (8.6 cm × 6.8 cm) 50 ml
Criterion (13.3 cm × 8.7 cm) 100 ml
PROTEAN II (16 cm × 16 cm) 250 ml
PROTEAN Plus (25.6 cm × 23 cm) 500 ml
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