Bio-Rad Fluorescent Protein Stains - Manual (Page 2)

Table of Contents
Section 1 Introduction and General Information........................1
1.1 Introduction.........................................................................1
1.2 Product Description ............................................................1
1.3 Storage ...............................................................................1
1.4 Materials and Equipment Required but not Supplied ..........2
1.5 Reagents Required but not Supplied ..................................2
1.6 Safety Considerations .........................................................2
1.7 Disposal Considerations......................................................2
1.8 Fluorescence Characteristics ..............................................2
Section 2 Instructions .....................................................................4
2.1 General Considerations.......................................................4
2.2 Prepare Fix Solution ............................................................4
2.3 Fix Gels...............................................................................4
2.4 Prepare Stain Solution ........................................................5
2.5 Stain Gels ...........................................................................5
2.6 Background Reduction (Optional) .......................................5
2.7 Gel Imaging.........................................................................5
Section 3 Technical Information....................................................6
3.1 Sensitivity with Laser Scanning ...........................................6
3.2 Sensitivity with UV Transillumination ....................................7
3.3 Protein-to-Protein Variability ................................................7
3.4 Linearity of Staining.............................................................8
Section 4 Troubleshooting ........................................................
Section 5 Product Information.......................................................9
4.1 Flamingo Fluorescent Gel Stain...........................................9
4.2 Related Products ................................................................9
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