Bio-Rad Fluorescent Protein Stains - Manual (Page 11)

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R 2 = 0.9943
0
500
1000
1500
2000
2500
0 1 2 3 4 5
fl
u
o
rescen
ce
R 2 = 0.9947
0
5000
10000
15000
20000
25000
0 10 20 30 40 50 60 70
ng β-galactosidase
fl
u
o
rescen
ce
R 2 = 0.9960
0
50000
100000
150000
200000
250000
300000
0 200 400 600 800 1000 1200
ng β-galactosidase
fl
u
o
rescen
ce
A
C
B
ng β-galactosidase
Fig. 4. Serial 2-fold dilutions of ββ-galactosidase, run on a Criterion™ Tris-HCl 4–20% gel.
The gel was stained with Flamingo Fluorescent Gel Stain and imaged with the Molecular Imager FX
system. Fluorescence associated with the β-galactosidase band was determined using Quantity One ®
1-D Analysis Software. A A: Fluorescence associated with protein band in the range 0.25–4 ng. B B:
Fluorescence associated with protein band in the range 4–60 ng. C C: Fluorescnece associated with protein
band in the range 60–960 ng.
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