Bio-Rad Fluorescent Protein Stains - Manual (Page 3)

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Section 1
Introduction and General Information
1.1 Introduction
Flamingo fluorescent gel stain allows sensitive fluorescence visualization of
proteins that have been separated by 1-D or 2-D SDS-PAGE. It comes as a 10x
stock solution that is diluted with water by the user to its working concentration. It
is supplied in three product sizes: 20 ml, 100 ml, and 500 ml.
Flamingo fluorescent gel stain contains a dye which binds to denatured protein.
This dye is normally non-fluorescent in solution, but becomes strongly fluorescent
when protein-bound. The staining procedure is a simple two-step protocol that
can be completed in as little as 5 hours. Gels to be stained are fixed with
ethanol/acetic acid solution prior to staining with the gel stain solution. A
destaining step is not normally required, but may be employed to reduce background.
Gels stained with Flamingo fluorescent gel stain may be visualized with a variety of
different fluorescence imaging systems. The most optimal imaging systems are
laser-based fluorescence scanners capable of exciting and detecting at 510 nm
and 535 nm respectively, such as the Molecular Imager FX
™ system. Gels may
also be imaged using systems that use 300 nm UV transillumination such as the
VersaDoc
™ imaging system.
Flamingo fluorescent gel stain gives exceptional sensitivity and dynamic range and
is compatible with subsequent analysis by enzymatic digestion and mass
spectrometry. It is thus particularly well-suited to proteomics applications. Stained
gels may be stored in the dark at 2–8°C for up to six months without significant
loss of imaging sensitivity.
1.2 Product Description
Flamingo fluorescent gel stain is a 10× stock solution that comes in three package
sizes. Instructions are included.
• The 20 ml size stains 4 mini gels (Ready Gel ® ) or 2 Criterion ™ gels.
• The 100 ml size stains 20 mini gels (Ready Gel), 10 Criterion gels, 4 PROTEAN ®
II gels, or 2 PROTEAN ® Plus gels.
• The 500 ml size stains 100 mini gels (Ready Gel), 50 Criterion gels, 20 PROTEAN II
gels, or 10 PROTEAN Plus gels.
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