Bio-Rad Fluorescent Protein Stains - Manual (Page 4)

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1.3 Storage
The product is stable for at least 6 months when stored at 2–8°C. Avoid exposure
to temperatures greater than 37°C and protect from light.
1.4 Materials and Equipment Required but not Supplied
• Staining containers—Glass trays are recommended, plastic trays may be used if
cleaned and rinsed thoroughly
• Imaging equipment—Gels are best imaged using a laser-based fluorescence
scanner capable of exciting and detecting near 510 nm and 540 nm respectively,
such as the Molecular Imager FX system. Gels may also be imaged using
systems based on the UV transilluminator and CCD camera such as the
VersaDoc imaging system
• Laboratory shaker or rocker
• Powder-free latex, vinyl or nitrile gloves
1.5 Reagents Required but not Supplied
• Acetic Acid, reagent grade
• Ethanol, reagent grade
• Filtered, distilled or deionized water.
1.6 Safety Considerations
Flamingo fluorescent gel stain is a dilute alcoholic solution of an organic dye. It is
flammable in its 10x concentrated form and should be handled in a manner that
prevents exposure to open flame or sparks. The diluted working solution is
minimally hazardous and non-flammable. However, the complete properties of the
dye component have not been investigated. Eye protection and gloves should be
worn and general laboratory safety precautions followed while handling both the
diluted and undiluted product.
1.7. Disposal Considerations
Laws governing the disposal of laboratory chemicals vary by region. Consult the
MSDS and check local laws for the proper disposal guidelines.
1.8. Fluorescence Characteristics
In the presence of protein, Flamingo fluorescent gel stain has a fluorescence
excitation maximum of 512 nm and a fluorescence emission maximum of 535 nm.
Flamingo fluorescent gel stain has a secondary excitation at 271 nm and can also
be excited with UV light (Fig. 1).
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