Bio-Rad Fluorescent Protein Stains - Manual (Page 10)

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3.2. Sensitivity with UV Transillumination
When scanned on the VersaDoc 4000 imaging system, an SDS-PAGE gel stained
with Flamingo fluorescent gel stain should exhibit a limit of sensitivity in the range
of 0.5–2 ng (see Fig. 3).
3.3. Protein-to-Protein Variability
Flamingo fluorescent gel stain will stain most proteins and exhibits less protein-to-
protein variability than other staining methods.
3.4. Linearity of Staining
Flamingo fluorescent gel stain allows linear quantitation over a wide dynamic
range. Staining with the standard 5 hour protocol (2 hour fix, 3 hour stain) will
allow linear quantitation from 1 to 100 ng. If the staining step is extended to
overnight (at least 12 hours), the linear range for quantitation can be extended to
over three orders of magnitude (0.5 to 1 µg).
Fig. 3. Serial 2-fold dilutions of SDS-PAGE molecular weight standards, broad range, on a
Criterion Tris-HCl 4–20% gel. The gel was stained with Flamingo fluorescent gel stain and imaged
with the VersaDoc 4000 imaging system using an exposure time of 5 min. The arrow indicates the lane
containing 0.5 ng of each standard.
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