Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 9)

9
Electroporation Conditions Are Known
Cell number <5 x 10 6
Exponential Waveform Protocols
Use preset protocols Opt mini 96-well/Exp to identify optimal voltage
and capacitance.
Gene Pulser MXcell Electroporation Guide
Check all well
sets after each
electroporation
for transfection
efficiency and
cell viability.
For an overview of
common analytical
methods, go to
pages 15–17.
Visit us on the Web at discover.bio-rad.com | Known Conditions: Exponential Setup — Mini
Choose the preset
mini protocols that
best match your
known conditions and
edit parameters as
needed, following the
instructions.
Good results? Congratulations! Be rewarded for your work and share your protocol
with scientists worldwide. Visit www.bio-rad.com/genetransferprotocols/.
1 2 3 4 5 6
A
200 V
0 µF 20 V
0 µF 00 V
0 µF 20 V
200 µF 20 V
0 µF 20 V
00 µF
B
C
D
Would you like to further optimize? For manual programming, go to page 14.
Keep the preset values for capacitance
and resistance:
n
Enter the known voltage value
(median voltage) in well set ABCD 2
n
Enter the median voltage decreased
by 0 V in well set ABCD 1
n
Enter the median voltage increased
by 0 V in well set ABCD
n
Enter the median voltage value in well
sets ABCD 4–6
n
Save the protocols under a new name
K
no
w
n
C
on
d
itio
ns
C
ell
#
<
5 x
1
0
6