Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 13)

1
Exponential and Square Waveforms Protocols
Use preset protocols Uniform 96-well/Exp, Sqr to identify optimal
conditions for the different cell lines.
Gene Pulser MXcell Electroporation Guide
Multiple Cell Lines
Check all well
sets after each
electroporation for
transfection efficiency
and cell viability.
For an overview of
common analytical
methods, go to
pages 15–17.
Good results? Congratulations! Be rewarded for your work and share your protocol
with scientists worldwide. Visit www.bio-rad.com/genetransferprotocols/.
You work with multiple
cell lines that require
both waveforms.
Use whole plate preset
protocols to deliver
exponential and
square waveforms.
Edit parameters as
needed, following the
instructions.
Use the left half of the plate to deliver exponential-decay pulses.
n
Enter your known voltage (median value) and vary capacitance around the known
value in well sets ABCD 1–6
n
Enter your known capacitance value and vary voltage around the median voltage
value in well sets EFGH 1–6
n
Use the preprogrammed setting for all other values
Use the right half of the plate to deliver square-wave pulses.
n
Enter your known median voltage value and vary pulse duration around the preset
value in well sets ABCD 7–12
n
Enter your known pulse duration or preset value and vary voltage around the median
voltage value in well sets EFGH 7–12
Save the protocols under a new name.
Exponential waveform Square waveform
1 2 3 4 5 6 7 8 9 10 11 12
A
20 V
0 µF 20 V
20 ms
B
C
D
E
F
G
H
Would you like to further optimize? For manual programming, go to page 14.
M
ult
ip
le
C
ell
Lin
es
Visit us on the Web at discover.bio-rad.com | Known Conditions: Multiple Cell Lines