Glossary – Bio-Rad Gene Pulser MXcell™ Electroporation System User Manual
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Electroporation plate:
a
plate in which electroporation is performed; available in
12-, 24-, and 96-well formats
Mini protocol:
a preprogrammed set of 4–6 different protocols delivered to 4–6 well sets
Parameters:
the physical constants (waveform, voltage, capacitance, duration,
resistance) that define the electric pulse
Preset protocols:
a set of preprogrammed protocols designed for rapid screening of
multiple parameters that can be used as a template
Protocol/electroporation conditions:
parameters defining the electric pulse
that will be delivered to specific well sets or wells on an electroporation plate
Waveform:
defines the type of electric pulse delivered to the cells
n
The exponential waveform builds up a charge in a capacitor and when the charge
is applied to the sample the voltage delivered decays exponentially, until the charge
remaining is about 7% of the original pulse
n
The square waveform relies on a charge being applied to the cells for a set time
Well set:
a set of four adjacent wells in a column on a 96-well plate; the same
electroporation conditions or protocol are applied to all the cells of a well set
Whole plate protocol:
a preprogrammed set of 24 protocols delivered across
the entire plate
Abbreviations
Cgrad:
capacitance gradient
D:
duration of pulse
Dgrad:
duration gradient
Exp:
exponential
NP:
number of pulses
P:
pulse
Sqr:
square
Vgrad:
voltage gradient
Schematic of 96-well
electroporation plate
One well set
Gene Pulser MXcell
Electroporation Guide
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