Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 17)

17
Fluorometer and Scanner Analysis for Adherent Cells
Fluorometric analysis of cell lysates can be used to examine lysed cells for the
presence of a fluorescence signal. This approach can be used for detecting
expression of a GFP-tagged protein.
Materials
n
Lysis buffer (0.% NP-40, 10 mM Tris pH .0, and 1 mM EDTA)
n
PBS
n
96-well dark plate with flat, clear bottom
Method
1. Remove the medium and wash the cells once with 00–700 μl of PBS.
2. Add 100 μl lysis buffer to each well and evenly distribute the lysis
buffer by gently rocking the plate.
. Incubate the plate at –0°C for 10 min.
4. Remove the plate from –0°C and allow the lysate to thaw on ice.
. Pipet each sample 4– times to wash cells off the bottom of the plate.
6. Transfer the sample to a 96-well dark plate with flat, clear bottom for
fluorometer or scanner analysis.
Gene Pulser MXcell Electroporation Guide
Electroporation Evaluation
Methods, continued
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