Bio-Rad Gene Pulser MXcell™ Electroporation System - Manual (Page 10)

Electroporation Conditions Are Known
Cell number <5 x 10 6
Gene Pulser MXcell Electroporation Guide
Check all well
sets after each
electroporation
for transfection
efficiency and
cell viability.
For an overview of
common analytical
methods, go to
pages 15–17.
Square Waveform Protocols
Use preset protocols Opt mini 96-well/Sqr to identify optimal voltage
and pulse duration.
Good results? Congratulations! Be rewarded for your work and share your protocol
with scientists worldwide. Visit www.bio-rad.com/genetransferprotocols/.
1 2 3 4 5 6
A
200 V
20 ms 20 V
20 ms 00 V
20 ms 20 V
1 ms 20 V
20 ms 20 V
2 ms
B
C
D
Would you like to further optimize? For manual programming, go to page 14.
Keep the preset values for pulse duration,
capacitance, and resistance:
n
Enter the known voltage value
(median voltage) in well set ABCD 2
n
Enter the median voltage decreased
by 0 V in well set ABCD 1
n
Enter the median voltage increased
by 0 V in well set ABCD
n
Enter the median voltage value in well
sets ABCD 4–6
n
Save the protocols under a new name
Visit us on the Web at discover.bio-rad.com | Known Conditions: Square Setup — Mini
Square Waveform Protocols
Use preset protocols Opt 96-well/Sqr, NP, D to identify optimal number of pulses.
1 2 3 4
A
20 ms
1 P 1 ms
2 P 10 ms
2 P 7 ms
P
B
C
D
Keep the preset values:
n
Enter the voltage that yielded the
best results from the
Opt mini
96-well/Sqr experiment
n
Save the protocols under a new name
For further optimization of your experiment, identify the optimal number of pulses.
Choose the preset
mini protocols that
best match your
known conditions and
edit parameters as
needed, following the
instructions.
10