Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 8)

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7.2 Components Required for Calibration .......................................................................... 111
7.3 Preparing Calibration Plates ........................................................................................ 112
7.4 Performing Calibrations .............................................................................................. 112
7.4.1 Performing Mask Alignment .................................................................................. 113
7.4.2 Performing Background Calibration ....................................................................... 114
7.4.3 Generating Persistent Well Factors........................................................................ 115
7.4.4 Performing Pure Dye Calibration ........................................................................... 116
7.4.5 Editing and Creating Pure Dye Plate Setups........................................................... 118
7.5 Viewing Calibration Files ............................................................................................. 119
7.6 Troubleshooting Optics with the Mask Image Window .................................................. 120
7.6.1 Filter Position....................................................................................................... 120
7.6.2 Camera Status ..................................................................................................... 120
Section 8. User Profiles ..............................................................................................122
8.1 User Preferences........................................................................................................ 122
8.1.1 File Paths Preferences .......................................................................................... 123
8.1.2 Plate Setup Preferences ....................................................................................... 124
8.1.3 Protocol Preferences ............................................................................................ 125
8.1.4 File Selection at Application Startup Preferences .................................................... 125
8.1.5 PCR Quant Screen Preferences ............................................................................. 126
8.1.6 Allelic Discrimination Module Preferences .............................................................. 127
8.1.7 End Point Module Preferences .............................................................................. 127
8.1.8 Gene Expression Module Preferences .................................................................... 128
8.2 User Administration.................................................................................................... 128
8.2.1 Adding New Users................................................................................................ 129
8.2.2 Deleting Existing Users......................................................................................... 130
8.3 Logging on to the iQ5 Software .................................................................................. 130
8.3.1 Switching Users ................................................................................................... 131
8.3.2 Changing Password.............................................................................................. 131
8.3.3 Defining Roles ..................................................................................................... 132
Section 9. Instrument Maintenance ...........................................................................133
9.1 Cleaning the Real-Time PCR Detection System............................................................. 133
9.2 Filter Description and Installation Instructions.............................................................. 133
9.2.1 Recommended Fluorophores and Filter Specifications............................................. 133
9.2.2 Accessing the Filters ............................................................................................ 134
9.2.3 Cleaning the Filters .............................................................................................. 134
9.3 Replacing the Lamp ................................................................................................... 135