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Bio-Rad iQ™5 Optical System Software, Version 2.1 User Manual

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Section 1 Getting Started

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iQ5 Optical Accessory Kit (catalog #170-9752)

Optical reaction block

Modified sliding rear cover for iCycler thermal cycler

Serial cable

USB cable

Filter extraction tool

Optical tape applicator tools (3)

Optics support bracket

Support bracket screws

Hex driver

Hex screws

Spare part: halogen lamp

MyiQ2 Calibrator Dye Solution Kit (catalog #170-8791)

1x calibration dye solutions (4 dyes, 3 tubes of each dye)

External Well Factor Solution (catalog #170-8794)

• External well factor solution (5 tubes)
Sample Consumables

• PCR plates and sealers

• Reagents

Contact your local Bio-Rad office if any system components are missing or damaged.

Accessories

The following accessories are required to complete the installation:

Scissors

#2 Phillips screwdriver

Calibrated micropipet(s)

Aerosol barrier pipet tips

Optical-quality sealing film or tube caps

Optical-quality PCR plates or tubes

1.2.2 Installing the Optical Reaction Module on the iCycler Chassis

1. Remove the existing rear cover from the iCycler chassis by sliding the cover towards the

front of the iCycler base.

2. Install the modified sliding rear cover provided with the optical reaction block, ensuring

that the notch is oriented towards the rear of the iCycler thermal cycler.

3. Push the sliding rear cover on top of the chassis as far back as possible.

4. Rotate the green latches on the optical reaction block up towards the open lid.

5. Lift the optical reaction block by the handle and install it onto the chassis. Lower the

front portion of the reaction block so that it engages with the chassis before the rear
portion. The rear of the block lid should fit over the front lip of the sliding rear cover.

6. Secure the optical reaction block in place by rotating the green latches downward.

7. Close the optical lid.