Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 149)

Appendices
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Cause:
Bad protocol file. There is no step defined for data collection or analysis in the thermal protocol.
Solution:
At least one data acquisition step must be present in the thermal protocol. Open the protocol
editor and specify a data collection at one step of the protocol. A data collection step is indicated
by a yellow (real-time data collection) or green (melt curve data collection) camera icon at the
appropriate step in the graphical display of the protocol. Refer to section 4.3 for further details.
Cause:
Fluorophores have been selected in the plate setup; however, no wells on the plate have been
edited to contain these fluorophores.
Solution:
This is a reminder to check your plate setup. Clicking No will return you to the Editing Plate
window. Clicking Yes will remove the unused fluorophores and save the plate setup.