Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 16)

Section 2 Quick Guides
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Section 2. Quick Guides
This section contains quick guides for the following topics:
o
Protocol Quick Guide (page 7)
o
Plate Setup Quick Guide (page 8)
o
Running a Real-Time Experiment Quick Guide (page 9)
o
Data Analysis Quick Guides
o
PCR Quant Tab Quick Guide (page 10)
o
End Point Quick Guide (page 11)
o
Allelic Discrimination Quick Guide (page 12)
o
Gene Expression Quick Guide (page 13)
o
Post-Run Plate Editing Quick Guide (page 16)
The fundamental steps of running a real-time PCR experiment on the MyiQ2, MyiQ, or iQ5 Real-
Time Detection System are the following:
1. Select, Edit, or Create a Protocol.
2. Select, Edit, or Create a Plate Setup.
3. Click Run
4. Select the appropriate Well Factor option and begin the run
5. Analyze the data
The quick guides below outline key procedures from each step. These quick guides are also
located in the online help of the iQ5 Optical System software.
For more complete information regarding the procedures and features of the iQ5 Optical System
software, refer to the relevant sections of this user manual.
2.1 Protocol Quick Guide
Within the Workshop module click the Protocol button.
Selecting a Protocol
1. Navigate to your desired protocol file using the file tree browser.
2. Click the protocol name once. The selected protocol appears in the Selected Protocol
pane.
NOTE: The iQ5 software has a number of sample protocol files which may be used.