Bio-Rad QX100™ Droplet Digital™ PCR System - Manual (Page 9)

QX100 Droplet Reader and QuantaSoft Software Instruction Manual QX100 Droplet Reader and QuantaSoft Software Instruction Manual | 3
Chapter 1 QX100 Droplet Digital PCR System
Table 1.1. QX100 Droplet Digital system components. Items shipped with the QX100 ddPCR system
(catalog #186-3001). Catalog # refers to replacement items (quantities may be different).
Component Description Catalog #
QX100 droplet generator
QX100 droplet generator
Instrument used for droplet generation 186-3002
DG8 droplet generator Microfluidic cartridge used to mix sample and oil to 186-3008
cartridges and gaskets (24) generate droplets; gaskets seal the cartridge to
prevent evaporation and apply pressure required
for droplet formation
Droplet generator cartridge Position and hold the droplet generator cartridge in 186-3051
holders (2) the instrument for droplet generation
Power cord Connects QX100 droplet generator to power source Call technical support
QX100 droplet reader
QX100 droplet reader
Instrument used for droplet reading, data collection 186-3003
Laptop PC and QuantaSoft software Connects to QX100 droplet reader for data collection 186-3007
and analysis
Droplet reader plate holders (2) Position 96-well PCR plate in the droplet reader 510-10608
USB 2.0 cable Connects QX100 droplet reader to PC Call technical support
Power cord Connects QX100 droplet reader to power source Call technical support
Table 1.2. Additional materials required.
Component Recommended Catalog # and Supplier
QX100 droplet generator
PCR supermix (required)
ddPCR supermix for probes 186-3010, 186-3026,
186-3027, 186-3028
One-Step RT-ddPCR kit for probes 186-3021, 186-3022
Control Buffer control kit 186-3052
Droplet generator oil Droplet generator oil (10 x 7 ml) 186-3005
Pipets 20 μl pipet for sample loading Rainin L-20
50 μl pipet for droplet transfer Rainin L-50, L8-50
8-channel, 200 μl pipet for oil Rainin L8-200
Pipet tips Filtered Rainin GP-L10F, GP-L200F
96-well PCR plates twin.tec semi-skirted 96-well plate Eppendorf 951020362
Reagent trough For droplet generator oil Thermo 95128095
Foil plate seals Easy Pierce foil plate seals Thermo AB-0757
Plate sealer Eppendorf 951023078
QX100 droplet reader
Droplet reader oil
Droplet reader oil (2 x 1 L) 186-3004
Droplet reader waste bottle Empty waste bottle N/A