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3 analyze – Bio-Rad QX100™ Droplet Digital™ PCR System User Manual

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Chapter 3 Using QuantaSoft Software

QX100 Droplet Reader and QuantaSoft Software Instruction Manual

3.3 Analyze

In the Setup window, load a plate (filename.qlp), then click Analyze to open and analyze the data. The data
analysis interface is separated into three windows:

Results table — summarizes results for wells selected in the well selector

Well selector — enables selection of wells for targeted analysis

Processed data/graphical display — allows visualization of graphical data from selected wells

Data analysis interface. Data from a CNV analysis are shown.

Graphical data
display

View table in graphical display window

Export data to .csv file

Select data from detector channel(s)

Toggle order in which channel data are displayed in the table

Display replicates separately, as merged wells (if Sample Name, Experiment, and
Assay Name and Type all match across the wells), or both

Results table options. Data from a CNV analysis are shown.

Results table

Well selector

Status options:

OK — automatic analysis successful

CHECK — automatic analysis unsuccessful; to

view concentration, use manual analysis
tools

Multi — data automatically analyzed as part of a

multi-well selection

Manual — droplets analyzed manually