Bio-Rad QX100™ Droplet Digital™ PCR System - Manual (Page 19)

| 13 QX100 Droplet Reader and QuantaSoft Software Instruction Manual
Chapter 3 Using QuantaSoft Software
QX100 Droplet Reader and QuantaSoft Software Instruction Manual
Well data stream
Channel 1 histogram
(events vs. amplitude)
Run indicator
Analyzed wells
3.2 Run
1. Click Run in the left navigation bar to start the run. After a few moments (up to 1 min), a green circle
appears next to the abort button and flashes periodically to indicate the run is in progress. Active and
analyzed wells are also highlighted in green in the plate map.
2. As each well is analyzed, the data appear across the top navigation area. Once the run is complete, all
data are reanalyzed for the final data file.
Switch data views
(charts to larger
window)
Interface during an active run. Data from all active detector channels are shown.
Experiment
Conc. channel 1 unknown
Conc. channel 2 unknown
Sample name
3.1.3 Using the Advanced Options
Click Options in the Setup window to see all advanced options for data collection and analysis.
Reprocess data with a newer version of the software
Export amplitude data for selected wells to a .csv file (one file for each well)
Advanced options.
Select wells by row (checked) or by column (unchecked)
Channel 1 (FAM) fluorescence vs. event number Channel 2 (VIC or HEX) fluorescence vs. event number
Channel 2 histogram (events vs.
amplitude)
Set chart options (threshold display)