Bio-Rad QX100™ Droplet Digital™ PCR System - Manual (Page 7)

QX100 Droplet Reader and QuantaSoft Software Instruction Manual | 1
QX100
™
Droplet Digital
™
PCR System
1
1.1 Introduction
The QX100 Droplet Digital PCR (ddPCR ™ ) system performs digital PCR with
unrivaled accuracy and precision. The system consists of two instruments, the
QX100 droplet generator and the QX100 droplet reader, and their associated
consumables (see Section 1.2). The QX100 droplet generator partitions samples
into 20,000 nanoliter-sized droplets, and after PCR on a thermal cycler, droplets
from each sample are analyzed individually on the QX100 droplet reader.
PCR-positive and PCR-negative droplets are counted to provide absolute
quantification of target DNA in digital form.
The ddPCR system lets you:
■ ■
Detect rare DNA target copies with unmatched sensitivity
■ ■
Determine copy number variation with unrivaled accuracy
■ ■
Measure gene expression levels with precision
Applications and uses include:
■ ■
Copy number variation
■ ■
Rare sequence detection
■ ■
Mutation detection
■ ■
Gene expression analysis
■ ■
miRNA analysis
■ ■
Next-generation sequencing sample quantification
This manual describes use of the QX100 droplet reader and QuantaSoft ™
software. For instructions on use of the QX100 droplet generator, please refer
to the QX100 Droplet Generator Instruction Manual (bulletin 10026322).