LaMotte 1200 Colorimeter - Manual (Page 62)

OZONE TEST PROCEDURE - INDIGO METHOD
Read the 1200 Colorimeter Manual before proceeding.
Carefully wipe tubes dry before inserting into the
colorimeter chamber.
PROCEDURE
2
Use the 1.0 mL
transfer pipet (2-
2170) and pipette
pump (2-2216) to
add 1.0 mL of HR
Reagent to each of
2 clean colorimeter
tubes (0967).
If chlorine is
present, add 3
drops Chlorine
Inhibitor (3990)
to each tube.
Cap tubes.
8.
5.
3. 4.
Use the 50 mL graduated
cylinder (0418) to carefully
add 45 mL of *Ozone Buffer
(3991) to amber glass bottle
marked HR Reagent (0680).
Use the 5 mL
volumetric pipet
(0329) and pipette
pump (2-2216) to add
5 mL of Indigo Blue
Stock Solution (3989)
to the amber glass
bottle. Cap and mix.
1. 2.
7.
Take one of the
prepared colorimeter
tubes and sampling
apparatus (0681) to
sampling site.
6.
Lower the end of the tubing
of sampling apparatus to
desired depth. Slowly
withdraw and depress
plunger several times to
purge syringe and tubing.
Slowly withdraw plunger to
fill purged syringe.
Remove plastic tubing
from syringe. Remove
Fill the second prepared
tube (0967) to the 10
mL line with ozone free
water. This is the
Reagent Blank.
HR Reagent
cap from the prepared tube.
Place tip of syringe against
inside of the prepared tube.
Slowly depress plunger and
fill to the 10 mL line and
cap. This is the Sample
Tube. NOTE: Do not shake
or invert the sample.