Copper ~ low range – LaMotte DC1600 Colorimeter User Manual
Page 31
COPPER ~ LOW RANGE
BICINCHONINIC ACID METHOD
CODE 3640
QUANTITY
CONTENTS
CODE
50
*Copper Tablets
*T-3808-H
*WARNING: Reagents marked with a * are considered hazardous substances. Material Safety Data Sheets (MSDS) are supplied for
these reagents. For your safety, read label and accompanying MSDS before using.
The copper content of drinking water generally falls below 0.03 parts per million, but copper levels as high as 1.0 parts per
million will give water a bitter taste. Waters testing as high as 1.0 part per million copper have probably been treated with a
copper compound, like those used in the control of algae, or have become contaminated from untreated industrial wastes.
The addition of copper sulfate to lakes causes an increase in the copper content of the sediments. Acid waters and those
high in free carbon dioxide may cause the corrosion or “eating away” of copper, brass and bronze pipes and fittings. This
corrosion results in the addition of copper into the water supply.
APPLICATION:
Drinking, surface, and saline waters; domestic and industrial wastes.
RANGE:
0 – 3.0 ppm Copper
METHOD:
Cupric ions form a purple complex with bicinchoninic acid around pH 6-7, in proportion to
the concentration of copper in the sample.
SAMPLE HANDLING
& PRESERVATION:
Copper has a tendency to be adsorbed to the surface of the sample container. Samples should
be analyzed as soon as possible after collection. If storage is necessary, 0.5 mL of 20% HCl per
100 mL of sample will prevent “plating out.” However, a correction must be made to bring the
reaction into optimum pH range.
INTERFERENCES:
High concentrations of oxidizing agents, calcium, and magnesium interfere. Silver can also
interfere.
PROCEDURE
1.
Rinse a clean colorimeter tube (0967) with sample water. Fill to the 10 mL line with sample.
2.
Select setting 5 on “Select Wavelength” knob and press “30 Second Read” button.
3.
Insert tube into colorimeter chamber and adjust to 100%T with “Set Blank” knob. This is the 100%T blank.
4.
Remove tube from colorimeter and add one *Copper Tablet (T-3808). Cap and shake vigorously until tablet dissolves.
Solution will turn purple if copper is present. Wait 2 minutes.
5.
At end of 2 minute waiting period, press “30 Second Read” button and insert tube into colorimeter chamber. Record
%T as soon as reading stabilizes.
6.
Consult calibration chart to determine copper concentration in parts per million (ppm).
DC1600 COPPER ~ LOW RANGE CALIBRATION CHART
%T
9
8
7
6
5
4
3
2
1
0
90
0.02
0.04
0.06
0.08
0.10
0.12
0.14
0.17
0.19
0.21
80
0.23
0.25
0.28
0.30
0.32
0.35
0.37
0.40
0.42
0.45
70
0.47
0.50
0.52
0.55
0.58
0.61
0.63
0.66
0.69
0.72
60
0.75
0.78
0.81
0.84
0.88
0.91
0.94
0.98
1.01
1.05
50
1.08
1.12
1.16
1.19
1.23
1.27
1.31
1.36
1.40
1.44
40
1.49
1.53
1.58
1.63
1.68
1.73
1.78
1.83
1.89
1.94
30
2.00
2.06
2.12
2.18
2.25
2.32
2.39
2.46
2.53
2.61
20
2.69
2.78
2.86
2.96
3.05
10
0
31