Metrohm 871 Advanced Bioscan - Manual (Page 37)

4.4 Faults and malfunctions
871 Advanced Bioscan / Instructions for Use 8.871.1003
29
Signal "overload" • Wrong IC-Net parameter • Check settings
'Range'
• Damaged working elec-
trode
• Flow cell is not con-
nected properly
easuring poten-
• Replace working electrode
• Check cable connections
(REF: black, WE: red,
AUX: blue)
• Optimize measuring po-
tential • Wrong m
tial
Considerable
press • Leak in system • Check connections and
ure drop make leakproof
Con
pre siderable
ssure rise
• Contamination of the
filter in the 6.2821.100 •
Filter unit PEEK
• Change of column pack-
ing by injection of con- • Regenerate the column
taminated samples
as been
used
Replace the 6.2821.110
Filter
(see section 4.2.1) or re-
place column
Note
• Mounting screws of flow
cell overtightened; 25
µm spacer h
:
s should always
microfiltered.
Sample
be
• Check screws of flow cell;
use 50 µm spacer
Chromatograms
with poor resolu-
tion, change in the
retention times • Deterioration in separa-
tion efficiency of the IC
column
• Old Eluent
• Ionic strength or pH of
the sample differs
strongly from eluent
• Regenerate the column
(see section 4.2.1) or re-
place column
• Replace eluent
• Dilute sample or change
pH of sample
Extreme peak
broadening,
splitting (double
peaks) • Dead volume at the col-
umn ends
• Dead volume in IC sys-
tem
• Replace column
• Check connections
No signal from
detector • No mains current
• 871 Advanced Bioscan
not switched on
• Faulty fuse
• Measuring cell not con-
nected
• Connection to IC Net is
not working properly
• Check mains supply and
voltage (see Section 2.2)
• Switch on the 871 at the
rear panel
• Replace fuse (see section
2.2.1)
• Check connection
• Check connection