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Metrohm 871 Advanced Bioscan User Manual

Page 35

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4.3 Maintenance and servicing

871 Advanced Bioscan / Instructions for Use 8.871.1003

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Also change the precolumn when you change the separation column.

1 Remove old column

Switch off flow cell.

Switch off pump drive of the 818 IC Pump.

Unscrew connection capillary 21 to flow cell from the column.

2 Connect new column to injector

Remove end caps from column.

Screw preheating capillary 31 to inlet end of separating col-

umn (note flow direction).

3 Rinse column

Place beaker beneath the column outlet.

Switch on 818 IC Pump and rinse column with eluent for ca.

10 min with 0.3 mL/min and for ca. 10 min. with 1.0 mL/min,

then switch off pump.

4 Connect column to measuring cell

Screw connection capillary 21 to measuring cell to outlet end


of separating column.