Figure 7, Representation of the scan of the pure eluent (b) – Metrohm 871 Advanced Bioscan User Manual
Page 28

3 Basic principles
871 Advanced Bioscan / Instructions for Use 8.871.1003
20
worry about contamination of your IC system you can still inject the
substance into the eluent using a large sample loop (> 500 µL). The
mple of a scan:
eluent should then be pumped at a very low flow rate (e.g. 0.2 mL/min).
Generally, same conditions should be used as they are needed for the
chromatographic separation (e.g. temperature, pH, eluent etc.).
Figure 7 s
Figure 7:
an interfering
be preferred in such cases.
nd the advan-
Example of a scan of a substance (A) with the additional repre-
sentation of the scan of the pure eluent (B)
When using metal electrodes as the working electrode, such as the
gold electrode used here, the reaction products formed by the am-
perometric detection during the scan method can form
layer on the electrode surface and influence the results. For this reason
a hydrodynamic voltammogram is to
The following table summarizes the requirements for a
tages and disadvantages of these two methods.
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