Metrohm 850 Professional IC Cation - LP-Gradient - Manual (Page 90)

6.1 Problems and their solutions
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850 Professional IC Cation – LP Gradient
Problem Cause Remedy
Sample – Gas bubbles in
the sample. Use the sample degasser (see Chapter 3.14,
page 44) (if available).
Very noisy baseline High pressure pump – con-
taminated pump valves. Clean pump valves (see "Cleaning the inlet
valve and outlet valve", page 67).
Eluent – Leak in the eluent
path. Check the eluent path
Eluent – Blockage in the
eluent path. Check the eluent path
High pressure pump –
defective piston seals. Replace piston seals (see "Replacing the piston
seal", page 64).
Pulsation damper not con-
nected. Connect the pulsation damper (see Chapter
3.13, Page 43).
Pulsation damper not con-
nected. Connect pulsation damper (see Chapter 3.13,
page 43).
Background conduc-
tivity too high Incorrect eluent. Change eluent (see Chapter 5.4.2.3, page 61).
Data of the separa-
tion column cannot
be read. Column chip contamina-
ted. Clean contact surfaces of the column chip
(with alcohol).
Column chip defective. 1. Save column configuration in MagIC Net.
2. Notify Metrohm Service.
Individual peaks
greater than expec-
ted Sample – Carry-over of the
samples from previous
measurement. Rinse system longer between two samples.
Poor reproducibility
of the retention
times Eluent – Leak in the eluent
path. Check the eluent path
Eluent – Blockage in the
eluent path. Check the eluent path
Chromatograms
have poor resolution Separation column –
diminished separating effi-
ciency. ￭ Regenerate separation column (see Chap-
ter 5.11.4, page 77).
￭ Replace separation column (see "Connect-
ing and rinsing the separation column",
page 54).