Metrohm 850 Professional IC Cation - LP-Gradient - Manual (Page 89)

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6 Troubleshooting
850 Professional IC Cation – LP Gradient
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6 Troubleshooting
6.1 Problems and their solutions
Problem Cause Remedy
Marked drop in
pressure Leak in the system. Check all capillary connections and seal leaks,
if necessary (see Chapter 3.5, page 17).
Marked rise in pres-
sure 6.2821.120 inline filter
blocked. Replace 6.2821.130 filter (see Chapter 5.6,
page 72).
Guard column – blocked. Replace guard column (see Chapter 3.18,
page 52).
Separation column –
blocked. ￭ Regenerate separation column (see Chap-
ter 5.11.4, page 77).
￭ Replace separation column (see "Connect-
ing and rinsing the separation column",
page 54).
Note: Samples should always be microfiltered
(see Chapter 5.7, page 74).
Injection valve – valve
blocked. Have the valve cleaned (by Metrohm service
technicians).
Drift of the baseline Thermal equilibrium not yet
attained. Condition (see Chapter 4.2, Page 56) instru-
ment with the column thermostat (see Chap-
ter 3.16, Page 48) switched on .
Leak in the system. Check all capillary connections and seal leaks,
if necessary (see Chapter 3.5, page 17).
Eluent – Evaporation of the
organic solvent in the elu-
ent. Check the eluent bottle attachment (see Figure
13, page 30).
Peak areas lower
than expected Sample – Leak in the sam-
ple path. Check the sample path.
Sample – Blockage in the
sample path. Check the sample path.
Sample – Sample loop not
(completely) filled. Prolong the sample transfer time.