Metrohm 850 Professional IC Cation - LP-Gradient - Manual (Page 24)

3.2 Initial installation
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850 Professional IC Cation – LP Gradient
￭ Connect the 6.2758.020 gradient mixing coil with its short pres-
sure screw directly to the input of the high pressure pump, (see
Chapter 3.11.2, page 39) and with its longer pressure screw to
the output of the mixing valve (see "Connecting mixing coil for
low pressure gradients", page 36).
￭ Screw on the 6.1834.120 connection tubing to the output of the
eluent degasser (see "Connecting the eluent degasser", page
33) and connect with input A of the mixing valve (see "Connect-
ing connection tubings", page 37).
￭ Equip two 6.1834.080 eluent aspiration tubings (see "Assembling
eluent aspiration tubing", page 28) and screw them tight to the
eluent degasser inputs B and C in the low pressure gradient.
6 Connecting the instrument
￭ Connect the instrument to the PC (see Chapter 3.17.1, page
51).
￭ Connect instrument to mains supply (see Chapter 3.17.2, page
51).
7 Initial start-up
See Chapter 4.1, Page 55
￭ Switch on the PC and start MagIC Net.
￭ Switch on and prepare the instrument.
￭ Deaerate the high pressure pump.
￭ Rinse the instrument without column(s).
8 Connecting the columns
￭ Remove the 6.2744.040 coupling between column inlet capillaries
and detector inlet capillaries.
￭ Connect guard column (optional) (see Chapter 3.18, page 52)
– Fasten the guard column to the end of the column inlet
capillary according to the specifications contained in the
leaflet accompanying the guard column.
– Rinse guard column.
￭ Connect the separation column (see Chapter 3.19, page 53)
– Fasten the inlet of the separation column either to the end
of the column inlet capillary or to the guard column (if
used) according to the specifications contained in the leaf-
let accompanying the column.
– Rinse separation column.
– Connect the detector inlet capillary and the output of the
separation column using a 6.2744.070 short PEEK pressure
screw.