SciGene Little Dipper Processor for FISH User Manual
Page 20
Little Dipper
®
Processor for FISH— USER MANUAL
March 2013
LD for FISH manual 2.0; software 1.4 for FISH
19
VII.
SYSTEM CALIBRATION
The instrument is calibrated and tested at the factory and should not require adjustment
under most circumstances.
A. Temperature
Controller Calibration
The EZ-Zone controllers are calibrated at the factory to
provide accurate bath temperatures from ambient +5 to
95°C (Fig. 42). Re-calibration is recommended only if the
display varies by more than one degree (1°C) to the
temperature shown on an externally connected NIST
certified digital thermometer (Cat. #1051-52-0).
To adjust a controller to achieve accurate temperatures:
1. Add water to bath fill line in a standard bath and ensure temperature sensor is
down in contact with liquid in the bath.
2. Turn ON the instrument.
3. Turn on the temperature controller and set the temperature on the controller to
65°C. Allow 30 minutes to stabilize.
4. Using the cable provided with the digital thermometer, plug one end into the blue
receptacle found above the controller.
5. Turn on the thermometer. Allow 1 minute for thermometer to stabilize. The actual
temperature of the bath will be displayed.
6. Calculate the difference between the temperature shown on the controller and on
the digital thermometer. For example, if the controller displays 65.0°C and the
thermometer displays 66.5°C, the difference (offset) is 1.5°C.
7. On the controller, press the up and down arrows simultaneously for 3 seconds. The
left display shows “A1” and the right display shows “open”.
8. Press the Advance Button (green circle) 3 times until the right display shows “i.CA”.
The left display shows the offset value between the controller and thermometer
when the unit was last calibrated.
9. Use the up or down arrow to adjust the offset value to the temperature difference
calculated in Step 4. For example, if the controller shows a temperature 1.5°C lower
than the thermometer, adjust the offset by adding 1.5 to the value shown.
10. Press the Infinity Key (∞) twice to exit calibration and return to the operation
display. Verify that the temperature on the thermometer matches the display. The
controller is now calibrated to provide accurate temperatures from 35 to 95°C.
Fig. 42. EZ Zone Controller