SciGene Little Dipper Processor for FISH - Manual (Page 14)

Little Dipper ® Processor for FISH— USER MANUAL
March 2013 LD for FISH manual 2.0; software 1.4 for FISH 13
F. Running a Pre-Installed Protocol
The instrument comes configured with validated protocols installed. Refer to the Little
Dipper Processor Method corresponding to your protocol for details on how to set up
the instrument. Method sheets can be found online at www.scigene.com.
Do not under any circumstances run a protocol while a bath cover is in place.
To achieve and maintain temperatures above 70˚C, a bath cover must be used,
while the machine is NOT in motion. Baths at high temperatures may need buffer(s)
added to offset evaporation.
The following are general guidelines on how to set up and run a pre-installed protocol.
1. Ensure baths, racks and stir bars are free of buffer residue and dust. See section
VI. A. Cleaning Before Every Use.
2. Insert baths and stir bars as specified for the protocol (Fig. 23).
3. Rotate all temperature sensors down (Fig. 24).
Fig. 23. Place baths and stir bars. Fig. 24. Rotate sensors down.
Fig. 25. Turn on main power. Fig. 26. Set temperature(s).
4. Add recommended buffer(s) to the fill line.
5. Turn on main power to the instrument (Fig. 25).
6. Turn on power to bath(s) to be heated and set temperature(s) (Fig. 26). Cover
any baths set above 70°C. Allow time for baths to reach set temperature(s).
7. Place a balance rack into the centrifuge with same number of slides as the
sample rack.
8. Add sample slides to rack.
9. Remove any bath covers.
10. Submerge the slide rack in Bath #1 between the two black lines.
11. On the touchscreen home page, press Run Protocol, then select the desired
protocol and press Start Now (Fig. 27).