SciGene Using Mai Tai Hybridization Cassettes - Manual

Pub. 406 Rev.E 0611
Mai Tai ® Hybridization System
SciGene ◊ 617 N. Mary Avenue ◊ Sunnyvale, California 94085 USA ◊ (408) 733-7337 [email protected]
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USER GUIDE
Equipment Needed
• Mai Tai ® Hybridization System*, 115v/230v.
(SciGene Cat. #1057-00-1/1057-00-2)
Includes a Model 777 Microarray Oven, a Mai Tai Rotator
and two Mai Tai Cassettes.
*The Mai Tai Rotator and Cassettes are compatible with
SciGene Series 700 Microarray Ovens (Models 777 and 700)
and Robbins Scientific Model 400 Ovens.
• Agilent or other microarrays compatible with Agilent gasket
slides on 1x3 inch (25x75mm) substrates.
• Agilent Hybridization Chamber gasket slides, 5-pack, that
match the microarray format used:
 Gasket slides, 1 x 244K microarrays
(Agilent cat. #G2534-60003)
 Gasket slides, 2 x 105K microarrays
(Agilent cat. #G2534-60002)
 Gasket slides, 4 x 44K microarrays
(Agilent cat. #G2534-60011)
Mai Tai ® Cassette Assembly
Using Mai Tai® Hybridization Cassettes
The cassette has a
standard microplate
footprint so samples may
be loaded manually or with
robotic liquid handling
equipment, such as the
ArrayPrep
® Target
Preparation System
[SciGene Cat. #2000-00-1
(115v); 2000-00-2 (230v)].
Place the gasket slide down
flat, ensuring the slide does
not rest on the edge of the
base plate (Figure 3). Practice
placing ordinary 1 x 3” (25x75mm) glass slides into the
chamber prior to running an experiment.
4. Pipette labeled sample into the center of each chamber of
the gasket slide using the volumes shown in Table 1.
Load samples onto all gasket slides BEFORE placing arrays.
Mai Tai ® Hybridization System
Using Mai Tai ® Hybridization Cassettes
1. With Mai Tai ® Rotator installed,
pre-heat the hybridization
oven to the desired
incubation temperature.
2. Place the base plate
on a flat surface.
3. Place a new gasket
slide into each cassette
chamber with the “Agilent”
label face up and aligned with
the “barcode” label (Figure 2).
Sample loss has been noted
with previously used slides.
The Mai Tai ® Cassette design uses just two parts that simplify
set up and disassembly and reduce the risk of errors (Figure 1).
Gasket
Format
Probe
Volume
1x microarray 490 µl
2x microarray 245 µl
4x microarray 100 µl
Table 1. Sample Volumes
Don’t touch
the gasket!
DISPENSE SAMPLE IN CENTER
OF GASKET COMPARTMENT
To prevent spilling or leaking, dispense hybridization
solution to the center of the gasket well without touching
the gasket with the pipette tip. Some sample will “wick”
over the barrier if it touches the gasket during assembly.