6 sectioning, Operation – Leica Biosystems SM2010 R User Manual
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Instructions for Use V 1.4 RevC – 09/2013
Cutting into the specimen (trimming)
For trimming, the specimen feed can be dis-
engaged either by turning the coarse driving
wheel (36) or by operating the manual feed
lever (35).
• Hold the knife sledge (11) at the grip (12) and
place the sledge behind the specimen.
• Pull the knife guard (14) of the blade/knife
holder to the right.
• To feed the specimen towards the knife, turn
the coarse driving wheel (36);
or
select the required section thickness with
the section thickness adjusting knob (33) and
move the manual feed lever (35). Each lever
movement causes a specimen feed by the
selected value.
• Move the knife sledge forth and back until the
specimen surface is trimmed as required.
5. Operation
5.6 Sectioning
Remove the sections
Always use different areas of the cutting edge for
trimming and sectioning.
• Select the required section thickness with the
section thickness adjusting knob (33).
• To produce the section, pull the knife sledge
over the specimen at a constant speed.
• Carefully remove sections using a small brush
and prepare them.
33
36
35
14
11
23
24
12
When using the automatic advance
feature, make sure to move the knife
sledge against the limit stop position to
disengage an automatic feed after each
section.
The coarse driving wheel must not be-
come locked! Otherwise there will be
no correct feed motion of the section
thickness.
Fig. 35