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6 sectioning, Operation – Leica Biosystems SM2010 R User Manual

Page 30

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Instructions for Use V 1.4 RevC – 09/2013

Cutting into the specimen (trimming)
For trimming, the specimen feed can be dis-

engaged either by turning the coarse driving

wheel (36) or by operating the manual feed

lever (35).
• Hold the knife sledge (11) at the grip (12) and

place the sledge behind the specimen.

• Pull the knife guard (14) of the blade/knife

holder to the right.

• To feed the specimen towards the knife, turn

the coarse driving wheel (36);

or

select the required section thickness with

the section thickness adjusting knob (33) and

move the manual feed lever (35). Each lever

movement causes a specimen feed by the

selected value.

• Move the knife sledge forth and back until the

specimen surface is trimmed as required.

5. Operation

5.6 Sectioning

Remove the sections
Always use different areas of the cutting edge for

trimming and sectioning.

• Select the required section thickness with the

section thickness adjusting knob (33).

• To produce the section, pull the knife sledge

over the specimen at a constant speed.

• Carefully remove sections using a small brush

and prepare them.

33

36

35

14

11

23

24

12

When using the automatic advance

feature, make sure to move the knife

sledge against the limit stop position to

disengage an automatic feed after each

section.

The coarse driving wheel must not be-

come locked! Otherwise there will be

no correct feed motion of the section

thickness.

Fig. 35