C.B.S. Scientific DGGEK-4801 - Manual (Page 2)

2 3 www.cbsscientific.com Cipher DGGE Instructions 2/28/14
IMPORTANT USER INFORMATION
This Instruction Manual will explain how to use this product safely and effectively.
Please read and carefully follow the instruction manual in its entirety.
The triangle/exclamation mark symbol alerts the user of the product to important opera-
tional, maintenance, and/or warranty requirements.
The triangle/lighting bolt symbol alerts the user of the product to potentially hazardous
electrical exposure.
Failure to adhere to the instructions could result in personal and/or laboratory hazards,
as well as invalidate any warranty. Always turn off the DC power source prior to discon-
necting power cords from the product. Disconnect power cords from the power source
first and then from the product. For maximum safety, always operate this system in an
isolated, low traffic area, not accessible to unauthorized personnel. Never operate dam-
aged or leaking equipment.
WARRANTY AND LIABILITY
This product was produced utilizing the highest practical standards of materials, work-
manship, and design. C.B.S. Scientific warrants that the product has been tested and
will meet or exceed published specifications. This warranty is valid only if the product
has been operated and maintained according to the instructions provided.
C.B.S. Scientific warrants this product to be free from defects in materials and work-
manship under normal service for one year from date of shipment. If the product
proves defective during this period, C.B.S. Scientific will repair or replace it at our
option, free of charge, if returned to us postage prepaid. This warranty does not
cover: damage in transit, damage caused by carelessness, misuse or neglect, normal
wear through frequent use, damage caused by solvent corrosion, damage caused by
improper handling or user alteration, nor unsatisfactory performance as a result of con-
ditions beyond our control. C.B.S. Scientific shall in no event be liable for incidental nor
consequential damages, including without limitation, lost profits, loss of income, loss
of business opportunities, loss of use and other related damages, however caused, nor
any damage arising from the incorrect use of the product.
ENGLISH T A B L E O F C O N T E N T S
Important User Information 3-4
Section 1: General Information
1 1 Introduction 5
1.2 Specifications 5
1.3 Safety 6
Section 2: DGGEK Unit Set-Up
2.1 Unpacking Instructions 6-7
2.2 Filling DGGEK Tank with Buffer 7
2.3 Heater/Stirrer/By-Pass Pump (Controller) Instructions 8-13
2.4 Setting Buffer Temperature 14
2.5 Connecting Power Supply 14-15
Section 3: Gel Casting Options
3.1 Preparation of Glass Plates 16
3.2 Gel Casting Techniques 16-20
3.3 Vertical Gradient Formation 21
3.4 Perpendicular Gel Casting 22-23
Section 4: Gel Running Preparations
4.1 Preparation of Cassettes 24
4.2 Buffer Cycling Set-Up 25-26
Section 5: Running Conditions
5.1 Running the Gels 26
5.2 Removing the Gels 27
5.3 Buffer Disposal 27
Section 6: Recommended Buffers
6.1 Stock Solutions 28
Section 7: Comb Specifications 29
Section 8: References 30
Section 9: Troubleshooting 31
Section 10: Maintenance of Equipment
10.1 Care and Handling 32
10.2 Maintenance 32
Section 11: Ordering Information 33
Section 12: Regulatory Compliance 34-35