C.B.S. Scientific DGGEK-4801 User Manual
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Cipher DGGE Instructions 2/28/14
SECTION 10
Maintenance of Equipment
10 1 Care and Handling
The plastic components of the DGGE Systems are fabricated from acrylic
and polycarbonate. Electrodes and connectors are made from pure platinum,
stainless steel, and chrome plated brass. As with any laboratory instrument,
adequate care ensures consistent and reliable performance.
After each use, rinse gel cassettes, spacers and combs with de-ionized
water. Wipe dry with a soft cloth or paper towel, or allow to air dry. Whenever
necessary, all components may be washed gently with water and a non-
abrasive detergent, and rinsed and dried as above. Never use abrasive
cleaners, glass cleaning sprays or scouring pads to clean the components, as
these will damage the unit and components.
Additional precautions:
• Do not autoclave or dry-heat sterilize the apparatus or
components.
• Do not expose the apparatus or components to phenol,
acetone, benzene, halogenated hydrocarbon solvents or
undiluted alcohol's.
• Avoid prolonged exposure of the apparatus or components to
UV light.
• Do NOT treat with diethylpyrocarbonate (DEPC)-treated water
for extended periods at 37°C. A brief rinse with DEPC-water
is sufficient after a thorough wash.
10 2 Maintenance
The following inspection and maintenance procedures will help maintain the
safety and reliable performance of the Cipher DGGEK systems. Replacement
parts can be ordered by calling 858-755-4959 or by contacting your local
distributor.
• Banana plugs and power cords should be inspected regularly.
If the banana plugs become loose or do not feel friction tight
replace the plugs or power cords.
• Should power cord assemblies (connectors, wire or shrouds)
show any signs of wear or damage (e.g. cracks, nicks,
abrasions, or melted insulation), replace them immediately.
• The platinum wire is secured to the banana jack by compression
between a stainless washer and the jack nut. The nut/washer
interface should be tight and free of corrosion.
SECTION 11
Ordering Information
CAT. #
DESCRIPTION
CIPHER DGGE ELECTROPHORESIS SYSTEMS
DGGEK-1001
Cipher DGGE Electrophoresis System, 2-place, 1 dual cassette, Specify 110V or 220V. Gel size 17.7cm
(w) x 22cm (l). Includes Electrophoresis tank, 1 dual cassette, heater/stirrer/buffer cycler, gradient maker,
4 combs (2 each 1 well and 2 ea 16 well combs), 4 sets of spacers, (2 sets for vertical DGGE and 2 sets
for perpendicular DGGE), 2 glass plate sets, 4 pieces gel wrap gasket, 12ea #2 clamps, and 18ea #1
clamps, glass safety cover, buffer tank drain valve, power leads, gel tape, buffer displacement tank, EPS-
300 X Power Supply and MPP-100 Mini-Perstaltic Pump. Please specify thickness of combs and spacers.
DGGEK-2001
Cipher DGGE Electrophoresis System, 2-place, 2 single cassettes, Specify 110V or 220V. Includes 2
single cassettes (instead of 1 dual cassette) and no buffer displacement tank, otherwise includes same as
DGGEK-1001.
DGGEK-2401
Cipher DGGE Electrophoresis System, 4-place, 2 dual cassettes, Specify 110V or 220V. Gel size 17.7cm
(w) x 22cm (l). Includes Electrophoresis tank, 2 dual cassettes, heater/stirrer/buffer cycler, gradient
maker, 8 combs (4 each 1 well and 4 ea 16 well combs), 8 sets of spacers, (4 sets for vertical DGGE and
4 sets for perpendicular DGGE), 4 glass plate sets, 8 pieces gel wrap gasket, 24ea #2 clamps, and 36ea
#1 clamps, glass safety cover, buffer tank drain valve, power leads, gel tape, EPS-300 X Power Supply
and MPP-100 Mini-Perstaltic Pump. Please specify thickness of combs and spacers.
DGGEK-4801
Cipher DGGE Electrophoresis System, 8-place, 4 dual cassettes, Specify 110V or 220V. Gel size 17.7cm
(w) x 22cm (l). Includes Electrophoresis tank, 4 dual cassettes, heater/stirrer/buffer cycler, gradient
maker, 16 combs (8 each 1 well and 8 ea 16 well combs), 16 sets of spacers, (8 sets for vertical DGGE
and 8 sets for perpendicular DGGE), 8 glass plate sets, 16 pieces gel wrap gasket, 48ea #2 clamps, and
72ea #1 clamps, glass safety cover, buffer tank drain valve, power leads, gel tape,EPS-300 X Power
Supply and MPP-100 Mini-Perstaltic Pump. Please specify thickness of combs and spacers.
Related Products
VGS0720RC-177
Spacer Set for Perpendicular DGGE, standard spacer + injection port spacer, 0.75mm thickness x 22cm(l)
VGS1020RC-177
Spacer Set for Perpendicular DGGE, standard spacer + injection port spacer, 1.0mm thickness x 22cm(l)
VGS1520RC-177
Spacer Set for Perpendicular DGGE, standard spacer + injection port spacer, 1.5mm thickness x 22cm(l)
VGC-0701-177
Comb for Perpendicular DGGE, 0.75mm thick x 1 well
VGC-1001-177
Comb for Perpendicular DGGE, 1.0mm thick x 1 well
VGC-1501-177
Comb for Perpendicular DGGE, 1.5mm thick x 1 well
VGS-0720R-177
Gel Wrap® Spacer Set, 0.75mm thick x 22cm with rounded bottom corners
VGS-1020R-177
Gel Wrap® Spacer Set, 1.0mm thick x 22cm with rounded bottom corners
VGS-1520R-177
Gel Wrap® Spacer Set, 1.5mm thick x 22cm with rounded bottom corners
VGE-0720-177
Gel Wrap® Gasket to cast one DGGE gel, 0.75mm thick
VGE-1020-177
Gel Wrap® Gasket to cast one DGGE gel, 1.0mm thick
VGE-1520-177
Gel Wrap® Gasket to cast one DGGE gel, 1.5mm thick
NGP-17720NR
Gel Wrap® Glass plate set, back plate has rounded bottom corners, 17.7cm (w) x 22 cm (h)
NGP-17720DNR
Gel Wrap® Glass plate set, Borosilicate back plate with rounded bottom corners, 17.7cm (w) x 22 cm (h)
NGP-17720-N-LPF
Gel Wrap® Glass Plate Set, both plates are Borosilicate. Back plate has rounded bottom corners, 17.7cm
(w) x 22 cm (h)
GCC-404-177K
Multiple Gel Casting Chamber Kit, 4-place, casts up to 4 each 15cm x 20cm gels
VGS-0720C-177
Multiple Gel Casting Spacer Set, 0.75mm thick x 22m(l)
VGS-1020C-177
Multiple Gel Casting Spacer Set, 1.0mm thick x 22m(l)
VGS-1520C-177
Multiple Gel Casting Spacer Set, 1.5mm thick x 22m(l)
NGP-17720-N
Multiple Gel Casting Chamber Glass Plate Set, 7.7cm(w) x 22cm(h)
NGP-17720-DN
Multiple Gel Casting Chamber Glass Plate Set, with Borosilicate back plate, 7.7cm(w) x 22cm(h)
NGP-17720-N-LPF
Multiple Gel Casting Chamber Glass Plate Set, both plates are Borosilicate, 7.7cm(w) x 22cm(h)