C.B.S. Scientific DGGEK-4801 - Manual (Page 13)

24 25 www.cbsscientific.com Cipher DGGE Instructions 2/28/14
SECTION 4
Gel Running Preparations
4 1 Preparation of the Cassettes
1. After polymerization rinse gel plate assembly with D.I. water to remove
excess acrylamide or denaturants from plate exterior.
2. Remove comb and quickly transfer gel
sandwich to cassette by removing all #2
clamps. LEAVE GEL WRAP IN PLACE
The Gel Wrap acts as a barrier and
prevents perpendicular electrical fields
from interfering with outside lanes. Re-
clamp to cassette using #1 clamps/4
per side. Dislodge bottom portion of
Gel Wrap from the plates to allow buffer
contact with the gel. See figure 4-1a.
NOTE: If running only 1 gel on a dual
cassette, attach conversion plate with
clamps to second side of cassette.
3. Immerse single (fig. 4-1b) or dual cassette(s) (fig. 4-1c) into pre-heated buffer
tank.
4 2 Buffer Cycling Connections
1. Fill upper reservoir(s) by attaching recirculating tubing to barb fitting on each
upper reservoir (fig. 4-2c,d,e). Rinse loading interface of gel with tank buffer
to remove non-polymerized acrylamide or excess urea. Adjust final level of
lower reservoir buffer by filling the tank until the level of buffer reaches the
underside of the upper reservoir.
Buffer Cycling Connections – Two Single Cassettes
Fig 4-1a
Fig 4-1b
Fig 4-1c
Fig 4-2c
Fig 4-2d